TrueFect^TM Transfection Reagent

- For DNA transfection

Catalog_No.	Product	Unit	Price
NF0866-3	TrueFect, Maximum strength for DNA in vitro tranfection, sufficient for 1500-3000 transfection (96 well plate)	1.0 mL	$169.00

Key Features:

• Highly effective in DNA transfection with low cytotoxicity
• Suitable for long DNA
• Applicable to many cell lines including sf9 cell
• Applicable to many cell lines
• Generate lentivirus with extremely high titer
• Stable formulation with long shelf life

Broad Transfection Spectrum for Mammalian Cell Types:

Cell Lines	Transfection Efficiency(% GFP)	Cell Lines	Transfection Efficiency(% GFP)
3LL	51%	K562	22%
B16-F10	72%	L929	52%
BAEC	52%	MCF-7	51%
BHK-21	86%	MDCK	52%
Ca Ski	78%	Neuro2A	72%
CaCo2	32%	NIH 3T3	88%
CHO	83%	PC12	34%
HCS-2/8	42%	SH-SY5Y	14%
HEK-293	80-90%	SiHa	62%
HeLa	81%	SKOV3	52%
HLMEC	50-65%	HUVEC	51%
H-MVEC	49%	IGROV1	21%
Huh-7D12	22%	Jurkat	5%-15%
ATT20	38%	6CSFMEo	73%
SK-N-SH	20%	WEHI 231	25%
McArdle 7777	60%	SAOS-2	52%
Hep3D	78%	SN56	69%
SHEP	71%	MC3T3-E1	80%
MDA-MB-231	38%	BT474	46%
3T3-442A	10%	C2C12	46%
COS-7	60-70%	Primary mouse keratinocytes	20-30%
CV-1	53%	Primary human pre-adipocytes	20-30%
D 407	62%	Primary human skin fibroblasts	15%
DHD Pro.b	51%	Primary mouse embryonic fibroblast	10%
LS180	46%	Primary melanocyte	15-20%
A549	68%	Hela-S3	78%

Primary Murine Skin Fibroblast transfected with pEGFP-C1 plasmid using TrueFectTM In Vitro DNA Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection