| Catalog #: | E02030 |
| Description: | 10X coating buffer. pH 9.5 |
| Buffer: | 10 mM bicarbonate, 50 mM NaCl at pH 9.5 (after dilution). |
| Storage: | Short term store at 2-8 oC to 6 months). |
| Instruction: | Make 10-fold dilution of the 10X assay diluent buffer (1 part buffer with 9 part of distilled or de-ionized water). Working solution (after dilution) can be stored at room temperature for up to 2 weeks. |
| Notes: | Higher pH normally induces some conformational changes of the antibody or antigen to allow more exposure of the hydrophobic regions of the protein, thus facilitating the non-specific binding to the solid surface. |
| Application: | This assay diluent can be used in alkaline phosphatase-based ELISA or blotting. |
| 10 mM bicarbonate, 50 mM NaCl at pH 9.5 (after dilution). |
| Unit: | 100mL |
| Price: | $80.00 |
